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Techniques

by vauterin — last modified 2012-01-04 17:58

 

Available techniques


  • Protein Research
    • protein purification (classical, FPLC, HPLC, expanded bed, monolith...)
    • polyclonal and monoclonal antibody production via protein- and via DNA-immunisation
         (anti GPIb, integrin alpha2-I, collagen, von Willebrand factor, ADAMTS13...)
    • various ELISA-formats: absorbance, fluorescence, luminescence
    • ristocetin cofactor assay in ELISA format
    • labeling (radioactive, biotin, HRP,...)
    • various (SDS-PAGE, western blot, IEF...)

  • Molecular biology
    • cell culture
    • vector construction, restriction analysis
    • mutagenesis: permeabilisation, PCR + pre-PCR room, electrophoresis, hybridisation...
    • humanisation monoclonal antibodies
    • yeast-two-hybrid
    • production recombinant proteins soluble (alpha2-I, VWF mutants, fragments, GPIb, ADAMTS13, antibodies, ...)
                                                     or surface expressed (alpha2-beta1, alphaIIb-beta3, WT & mutants: CHO-cells)

  • Phage display technology
    • linear 6-, 15-mer library, cyclic 6-mer polyvalent on pIII
    • landscape on pVIII

  • Cel biology
    • cell adhesion under static conditions (ELISA)
    • cell adhesion under dynamic flow conditions (flow chambers, online/off line image analysis)
    • platelet aggregation (in plasma, in buffer)
    • ligand & antibody-binding studies: ELISA, FACS, RA.
    • cell counter

  • Animal models
    • KO mice:                                VWF; ADAMTS13;  DCBLD2;  LRRC32-lox; 
      immune compromised mice:  NOD-SCID; NOD.SCID.IL2Rgamma;  IL-10/recombination-activating gene (RAG)2
    • von Willebrand disease dogs
    • gene transfer: hydrodynamic, lentiviral (L2 facility)



    • Mice FeCl3 mesenteric artery  thrombosis model: intravital fluorescence microscopy with digital image analysis
    • Mice stimulated endothelial VWF-platelet string model
    • Mice stroke model
    • NOD-SCID mice: in vivo production of human platelets from stem cells


    Are you interested in one of the above, please contact us
                                                                                   Hans.Deckmyn@kuleuven-kortrijk.be
                                                                                   Karen.Vanhoorelbeke@kuleuven-kortrijk.be

 
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